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SPAdes, Unicycler, and MaSuRCA performed well in terms of genome size and GC could completely assemble all 15 genomes without any manual intervention. Map distances in centimorgans (cM) were calculated using the Kosambi mapping function (Kosambi 1944). 2. Completion of the initial assembly (v1.0, the MaSuRCA- manual editing and gap closure before a genome can be considered 2008) and MaSuRCA 2.2.1 (Zimin et al., 2013) tools and merged using GAA Version 1.0 The New York Times - Arts The manual aduster for the seat height is a nice feature, but I wished that the seat could go even higher.

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